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T. Bohn and T. Walczyk (2004)

Determination of chlorophyll in plant samples by liquid chromatography using zinc-phthalocyanine as an internal standard

Journal of Chromatography A, 1024(1-2):123-128.

Chlorophyll analysis at high precision and accuracy is limited by the lack of suitable, commercially available internal standards for HPLC analysis. Here, the commercially available dye zinc-phthalocyanine is presented as a new internal standard to quantify chlorophylls in vegetable foods and to detect chlorophyll degradation products. The technique was applied to chlorophyll analysis of a selection of vegetable foods. Pigments were extracted with N,N-dimethylformamide from the vegetables and purified by solid phase extraction. Chlorophyll a, a', b, b', corresponding pheophytins, and zinc-phthalocyanine were separated by HPLC using a C-18 reverse-phase column and fluorescence detection. (C) 2003 Elsevier B.V. All rights reserved.

derivatives, chlorophylls, extracts, zinc-phthalocyanine, phytoplankton, quantification, identification, performance, carotenoid-pigments, degradation products, vegetables, hplc analysis
WOS:000187636600015
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